The AAA-ATPase p97 in mitosis and fertilization

Heubes, S. and Stemmann, O. (2007). The AAA-ATPase p97-Ufd1-Npl4 is required for ERAD but not for spindle disassembly in Xenopus egg extracts. Securin is not required for chromosomal stability in human cells. PLoS Biol 3, e416. Contents CONTENTS 1 SUMMARY 1 2 INTRODUCTION 3 2.1 The eukaryotic cell cycle – An overview 3 2.1.1 Establishment and resolution of cohesion between sister chromatids 4 2.1.2 Regulation of separase 5 2.1.3 Anaphase and the spindle assembly checkpoint 7 2.1.4 Assembly and disassembly of the mitotic spindle 9 2.2 The AAA-ATPase p97 10 2.3 Fertilization 13 2.4 The African clawed frog as model organism 14 2.5 Generation of dominant-negative p97 mutants 26 3.1.2 p97 is not required for spindle disassembly at the end of mitosis 27 3.1.2.1 p97-Ufd1-Npl4 interacts with tubulin 27 3.1.2.2 Depletion of p97 adaptors has no effect on spindle disassembly 28 3.1.2.3 Dominant-negative p97 mutants do not interfere with spindle disassembly 30 3.1.2.4 Establishment of ERAD in Xenopus egg extract 32 3.1.2.5 Spindle disassembly is not delayed in the absence of p97 function 34 3.1.2.6 Dispensability of p97 is independent of sperm chromatin 36 3.1.3 p97 and separase activation and stability 38 3.1.3.1 p97 is not required for securin degradation in Xenopus 38 3.1.3.2 p97 does not act as a chaperone for vertebrate separase 41 3.